Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit

Piper S, Edwards M, van Wonderen J, Casadevall C, Martel A, Jeuken L, Reisner E, Clarke T, Butt J, Frontiers in Microbiology 12 (2021) DOI

SASDL97 – Ru-MtrCAB complex formed by reconstitution of Ru-MtrC and MtrAB

Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA
Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC
Extracellular iron oxide respiratory system outer membrane component MtrB
MWI(0) 208 kDa
MWexpected 191 kDa
VPorod 154 nm3
log I(s) 3.62×10-1 3.62×10-2 3.62×10-3 3.62×10-4
Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC Extracellular iron oxide respiratory system outer membrane component MtrB small angle scattering data  s, nm-1
ln I(s)
Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC Extracellular iron oxide respiratory system outer membrane component MtrB Guinier plot ln 3.62×10-1 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC Extracellular iron oxide respiratory system outer membrane component MtrB Kratky plot 1.104 0 √3 sRg
p(r)
Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC Extracellular iron oxide respiratory system outer membrane component MtrB pair distance distribution function Rg: 4.8 nm 0 Dmax: 16.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC Extracellular iron oxide respiratory system outer membrane component MtrB DAMMIN model
SANS data from solutions of the Ru-MtrCAB complex formed by reconstitution of Ru-MtrC and MtrAB in 20 mM HEPES, 100 mM NaCl, 2.8 mM Fos-choline 12, 13% D2O, pD 7.8, were collected using the D22 small-angle neutron scattering diffractometer (Institut Laue-Langevin, ILL; Grenoble, France) equipped with a Reuter-Stokes 3He detector at a sample-detector distance of 17.6 m and at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.30 mg/ml was measured at 11°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA (MtrA)
Mol. type   Protein
Organism   Shewanella oneidensis (strain MR-1)
Olig. state   Monomer
Mon. MW   38.6 kDa
 
UniProt   Q8EG35 (35-333)
Sequence   FASTA
 
Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC (Ru-MtrCSII)
Mol. type   Protein
Organism   Shewanella oneidensis (strain MR-1)
Olig. state   Monomer
Mon. MW   76.8 kDa
 
UniProt   Q8EG34 (26-671)
Sequence   FASTA
 
Extracellular iron oxide respiratory system outer membrane component MtrB (MtrB)
Mol. type   Protein
Organism   Shewanella oneidensis (strain MR-1)
Olig. state   Monomer
Mon. MW   75.5 kDa
 
UniProt   Q8CVD4 (22-697)
Sequence   FASTA