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Synchrotron SAXS
data from solutions of
Ectodomain of human syndecan-4 isoform-2 Monomer
in
10 mM HEPES, 150 mM NaCl, pH 7.4
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a Eiger 4M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample
at 8.3 mg/ml was injected at a 0.20 ml/min flow rate
onto a GE Superdex 200 Increase 5/150 column
at 20°C.
600 successive
0.990 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
50 ul of the ectodomain of human syndecan-4 isoform-2 (8.3 mg/ml, comprising a mix of monomers and dimers) were injected on a Superdex S200 Increase 5/150 GL (GE Healthcare) at a flow rate of 0.2 ml/min at 20°C. Experimental molecular weight has been determined by ESI-MS (and in solution by SEC-MALLS, estimated at 18.0 kDa).
PED: https://proteinensemble.org/PED00188
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s, nm-1
Rg, nm
