Identification of a Region in the Common Amino-terminal Domain of Hendra Virus P, V, and W Proteins Responsible for Phase Transition and Amyloid Formation

Salladini E, Gondelaud F, Nilsson J, Pesce G, Bignon C, Murrali M, Fabre R, Pierattelli R, Kajava A, Horvat B, Gerlier D, Mathieu C, Longhi S, Biomolecules 11(9):1324 (2021) DOI

SASDLF9 – 200-310 region of Hendra virus P/V/W protein (PNT3)

Protein W
MWI(0) 18 kDa
MWexpected 15 kDa
VPorod 38 nm3
log I(s) 9.34×10-3 9.34×10-4 9.34×10-5 9.34×10-6
Protein W small angle scattering data  s, nm-1
ln I(s)
Protein W Guinier plot ln 9.34×10-3 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Protein W Kratky plot 1.104 0 √3 sRg
p(r)
Protein W pair distance distribution function Rg: 3.7 nm 0 Dmax: 15.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
200-310 region of Hendra virus P/V/W protein (PNT3) Rg histogram Rg, nm
Protein W PED (PROTEIN ENSEMBLE DATABASE) model
Protein W EOM/RANCH model
Protein W EOM/RANCH model
Protein W EOM/RANCH model
Protein W EOM/RANCH model
Protein W EOM/RANCH model
Synchrotron SAXS data from solutions of 200-310 region of Hendra virus P/V/W protein (PNT3) in 50 mM sodium phosphate, 5 mM EDTA, pH 6.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample at 3.5 mg/ml was injected at a 0.20 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 600 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Fit verified using CorMap PED: https://proteinensemble.org/PED00203

Tags: idp PED
Protein W (PNT3)
Mol. type   Protein
Organism   Hendra virus (isolate Horse/Autralia/Hendra/1994)
Olig. state   Monomer
Mon. MW   15.1 kDa
 
UniProt   P0C1C6 (200-310)
Sequence   FASTA
 
PED ID   PED00203