| MWexperimental | 8 | kDa |
| MWexpected | 8 | kDa |
| VPorod | 19 | nm3 |
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log I(s)
9.81×10-3
9.81×10-4
9.81×10-5
9.81×10-6
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s, nm-1
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Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2.
Sethi A, Bruell S, Ryan T, Yan F, Tanipour MH, Mok YF, Draper-Joyce C, Khandokar Y, Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR, J Mol Biol 433(21):167217 (2021) Europe PMCSASDLG7 – LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94)
Relaxin receptor 1|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of the LDLa linker region of RXFP1 (23-94) in 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH 7.4 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.4 m and at a wavelength of λ = 0.10332 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS with sheath-cell co-flow was employed. The SEC parameters were as follows: A 50.00 μl sample at 5 mg/ml was injected at a 0.40 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 21.8°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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