Synchrotron SAXS data from solutions of Human potassium voltage-gated channel (Kv2.1) T1 domain in buffer (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.01% Triton X-100, 10 mM BME) were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS) (Chicago, IL, USA) using a Pilatus3 X 1M detector (Dectris) at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-SAXS was employed. The SEC parameters were as follows: A 250 μl sample at 1.5 mg/ml was injected at a 0.50 ml/min flow rate onto a Superdex 200 increase column (GE healthcare) at 22°C. 2400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
