Structure and function of Bartonella effector protein 1: target and interdomain interactions

Markus Huber, Jens Reiners, University of Basel PhD thesis 15051 (2023) URL

SASDLK7 – Bartonella effector protein (Bep1), 15 °C

Bartonella effector protein (Bep) substrate of VirB T4SS
MWI(0) 60 kDa
MWexpected 64 kDa
VPorod 83 nm3
log I(s) 4.33×10-2 4.33×10-3 4.33×10-4 4.33×10-5
Bartonella effector protein (Bep) substrate of VirB T4SS small angle scattering data  s, nm-1
ln I(s)
Bartonella effector protein (Bep) substrate of VirB T4SS Guinier plot ln 4.33×10-2 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Bartonella effector protein (Bep) substrate of VirB T4SS Kratky plot 1.104 0 √3 sRg
p(r)
Bartonella effector protein (Bep) substrate of VirB T4SS pair distance distribution function Rg: 4.0 nm 0 Dmax: 13.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Bartonella effector protein (Bep) substrate of VirB T4SS GASBOR model
SAXS data from solutions of Bep1 in 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.5 were collected using Xenocs Xeuss 2.0 Q-Xoom instrument (Center for Structural Studies, Heinrich-Heine-University, Düsseldorf, Germany) equipped with a Pilatus3 R 300K detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 12.00 mg/ml was measured at 15°C. 30 successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Bartonella effector protein (Bep) substrate of VirB T4SS (Bep1)
Mol. type   Protein
Organism   Bartonella clarridgeiae (strain CIP 104772 / 73)
Olig. state   Monomer
Mon. MW   63.7 kDa
 
UniProt   E6YFW2 (2-558)
Sequence   FASTA