| MWexperimental | 94 | kDa |
| MWexpected | 87 | kDa |
| VPorod | 175 | nm3 |
|
log I(s)
5.07×10-2
5.07×10-3
5.07×10-4
5.07×10-5
|
s, nm-1
|
Autism associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher M, Niebling S, Han Y, Molodenskiy D, Nia FH, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M, Elife 10 (2021) Europe PMCSASDLL3 – SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (R12C)
SH3 and multiple ankyrin repeat domains protein 3|
|
|
Fits and models
|
|
|
|
|
Synchrotron SAXS
data from solutions of
SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (R12C)
in
100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH 6.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.5 and 8 mg/ml were measured
at 10°C.
20 successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
|
|
|||||||||||||||||||||||||||