| MWI(0) | 59 | kDa |
| MWexpected | 64 | kDa |
| VPorod | 87 | nm3 |
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log I(s)
4.56×10-2
4.56×10-3
4.56×10-4
4.56×10-5
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s, nm-1
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Structure and function of Bartonella effector protein 1: target and interdomain interactions
Markus Huber, Jens Reiners, University of Basel PhD thesis 15051 (2023) URLSASDLL7 – Bartonella effector protein (Bep1), 20 °C
Bartonella effector protein (Bep) substrate of VirB T4SS|
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Fits and models
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Synchrotron SAXS data from solutions of Bep1 in 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.80 mg/ml was measured at 20°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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