| MWexperimental | 114 | kDa |
| MWexpected | 108 | kDa |
| VPorod | 170 | nm3 |
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log I(s)
2.69×102
2.69×101
2.69×100
2.69×10-1
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s, nm-1
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Structural insights of RmXyn10A - A prebiotic-producing GH10 xylanase with a non-conserved aglycone binding region.
Aronsson A, Güler F, Petoukhov MV, Crennell SJ, Svergun DI, Linares-Pastén JA, Nordberg Karlsson E, Biochim Biophys Acta Proteins Proteom 1866(2):292-306 (2018) Europe PMCSASDLM4 – Prebiotic-producing GH10 xylanase (RmXyn10A, full length)
Prebiotic-producing GH10 xylanase (RmXyn10A, full length)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Prebiotic-producing GH10 xylanase (RmXyn10A, full length)
in
50 mM TRIS, 0.5 M NaCl, pH 7.4
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a 1D Gas detector detector
at a sample-detector distance of 2.3 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.5 and 13.7 mg/ml were measured
at 20°C.
15 successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Storage temperature = UNKNOWN
Tags:
X33
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