Synchrotron SAXS
data from solutions of
Interleukin 11 signalling complex, full-length extracellular gp130
in
20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH 8.5
were collected
on the
SAXS/WAXS beam line
at the Australian Synchrotron storage ring
(Melbourne, Australia)
using a Pilatus3 S 2M detector
at a sample-detector distance of 2.2 m and
at a wavelength of λ = 0.108 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 2 mg/ml was injected at a 0.40 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
Eight successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection volume = UNKNOWN
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