Protein quaternary structures in solution are a mixture of multiple forms

Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G, Chemical Science 13(39):11680-11695 (2022) DOI

SASDLQ4 – Deoxyribose-phosphate aldolase, DeoC

Deoxyribose-phosphate aldolase
MWI(0) 50 kDa
MWexpected 28 kDa
VPorod 61 nm3
log I(s) 3.61×10-2 3.61×10-3 3.61×10-4 3.61×10-5
Deoxyribose-phosphate aldolase small angle scattering data  s, nm-1
ln I(s)
Deoxyribose-phosphate aldolase Guinier plot ln 3.62×10-2 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Deoxyribose-phosphate aldolase Kratky plot 1.104 0 √3 sRg
p(r)
Deoxyribose-phosphate aldolase pair distance distribution function Rg: 2.6 nm 0 Dmax: 8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Deoxyribose-phosphate aldolase PDB (PROTEIN DATA BANK) model
Deoxyribose-phosphate aldolase PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Deoxyribose-phosphate aldolase PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Deoxyribose-phosphate aldolase, DeoC. in 50 mM HEPES, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.08 mg/ml was measured at 20°C. 40 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Deoxyribose-phosphate aldolase (DeoC)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Unknown
Mon. MW   27.7 kDa
 
UniProt   P0A6L0 (1-259)
Sequence   FASTA
 
PDB ID   1KTN
 
PDB ID   1KTN
 
PDB ID   1KTN