| MWexperimental | 88 | kDa |
| MWexpected | 85 | kDa |
| VPorod | 160 | nm3 |
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log I(s)
3.28×10-2
3.28×10-3
3.28×10-4
3.28×10-5
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s, nm-1
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Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M, Nature Communications 14(1) (2023) DOISASDLS3 – Interleukin 11 Mutein, complex with gp130 (domains 1-3) and IL-11Rα
Interleukin-11 receptor subunit alphaInterleukin 11 Mutein
Interleukin-6 receptor subunit beta
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Fits and models
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Synchrotron SAXS
data from solutions of
Interleukin 11 Mutein, complex with gp130 (domains 1-3) and IL-11Rα
in
20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH 8.5
were collected
on the
SAXS/WAXS beam line
at the Australian Synchrotron storage ring
(Melbourne, Australia)
using a Pilatus3 S 2M detector
at a sample-detector distance of 3.5 m and
at a wavelength of λ = 0.108 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 2 mg/ml was injected at a 45.00 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
13 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection volume = UNKNOWN |
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