Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis.

King-Scott J, Konarev PV, Panjikar S, Jordanova R, Svergun DI, Tucker PA, Structure 19(1):56-69 (2011) Europe PMC

SASDLS5 – Multidomain Regulatory Protein Rv1364c (dimer)

Multidomain regulatory protein Rv1364c
MWexperimental 123 kDa
MWexpected 139 kDa
VPorod 289 nm3
log I(s) 2.64×102 2.64×101 2.64×100 2.64×10-1
Multidomain regulatory protein Rv1364c small angle scattering data  s, nm-1
ln I(s)
Multidomain regulatory protein Rv1364c Guinier plot ln 2.64×102 Rg: 4.5 nm 0 (4.5 nm)-2 s2
(sRg)2I(s)/I(0)
Multidomain regulatory protein Rv1364c Kratky plot 1.104 0 √3 sRg
p(r)
Multidomain regulatory protein Rv1364c pair distance distribution function Rg: 5.3 nm 0 Dmax: 18 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Multidomain regulatory protein Rv1364c GASBOR model
Synchrotron SAXS data from solutions of Multidomain Regulatory Protein Rv1364c (dimer) in 25 mM Tris-HCl, 50 mM NaCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.3 and 6.7 mg/ml were measured at 20°C. One 180 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Multidomain regulatory protein Rv1364c (Rv1364c)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Dimer
Mon. MW   69.5 kDa
 
UniProt   P9WLZ7 (1-653)
Sequence   FASTA