A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence.

McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR, Nat Commun 13(1):4105 (2022) Europe PMC

SASDLS7 – Mycobacterium phage TipsytheTRex immunity repressor bound to DNA

Immunity repressor
21mer dsDNA
MWexperimental 30 kDa
MWexpected 34 kDa
VPorod 41 nm3
log I(s) 4.18×101 4.18×100 4.18×10-1 4.18×10-2
Immunity repressor 21mer dsDNA small angle scattering data  s, nm-1
ln I(s)
Immunity repressor 21mer dsDNA Guinier plot ln 4.19×101 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Immunity repressor 21mer dsDNA Kratky plot 1.104 0 √3 sRg
p(r)
Immunity repressor 21mer dsDNA pair distance distribution function Rg: 2.3 nm 0 Dmax: 7.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Immunity repressor 21mer dsDNA BILBOMD model
Synchrotron SAXS data from solutions of the Mycobacterium phage TipsytheTRex immunity repressor bound to DNA in 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Immunity repressor
Mol. type   Protein
Organism   Mycobacterium phage TipsytheTRex
Olig. state   Monomer
Mon. MW   21.4 kDa
 
UniProt   A0A2D1GKF7 (1-183)
Sequence   FASTA
 
21mer dsDNA
Mol. type   DNA
Olig. state   Monomer
Mon. MW   13.0 kDa
Sequence   FASTA
 
PDB ID   7R6R