Structure and dynamics of the von Willebrand Factor C6 domain

Emma-Ruoqi Xu.

SASDLT9 – von Willebrand factor C6-CK G2705R

von Willebrand factor C6-CK G2705R
MWexperimental 40 kDa
MWexpected 42 kDa
VPorod 95 nm3
log I(s) 5.23×10-2 5.23×10-3 5.23×10-4 5.23×10-5
von Willebrand factor C6-CK G2705R small angle scattering data  s, nm-1
ln I(s)
von Willebrand factor C6-CK G2705R Guinier plot ln 5.23×10-2 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
von Willebrand factor C6-CK G2705R Kratky plot 1.104 0 √3 sRg
p(r)
von Willebrand factor C6-CK G2705R pair distance distribution function Rg: 3.8 nm 0 Dmax: 16.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of von Willebrand factor C6-CK, G2705R mutant, in 20 mM HEPES, 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 27 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

von Willebrand factor C6-CK G2705R (VWFC6-CK G2705R)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   20.8 kDa
 
UniProt   P04275 (2647-2813)
Sequence   FASTA