| MWI(0) | 36 | kDa |
| MWexpected | 46 | kDa |
| VPorod | 254 | nm3 |
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log I(s)
3.86×10-2
3.86×10-3
3.86×10-4
3.86×10-5
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s, nm-1
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The Disease Associated Tau35 Fragment has an Increased Propensity to Aggregate Compared to Full-Length Tau
Lyu C, Da Vela S, Al-Hilaly Y, Marshall K, Thorogate R, Svergun D, Serpell L, Pastore A, Hanger D, Frontiers in Molecular Biosciences 8 (2021) DOISASDLU4 – Tau protein, 2N4R isoform
Microtubule-associated protein tau, 2N4R isoform|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of the 2N4R isoform of Tau protein in phosphate buffered saline, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Tags:
idp
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