Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation

Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J, npj Biofilms and Microbiomes 8(1) (2022) DOI

SASDLW2 – Accessory colonization factor SslE at pH 4.4

Accessory colonization factor
MWexperimental 158 kDa
MWexpected 160 kDa
VPorod 248 nm3
log I(s) 2.06×10-1 2.06×10-2 2.06×10-3 2.06×10-4
Accessory colonization factor small angle scattering data  s, nm-1
ln I(s)
Accessory colonization factor Guinier plot ln 2.06×10-1 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Accessory colonization factor Kratky plot 1.104 0 √3 sRg
p(r)
Accessory colonization factor pair distance distribution function Rg: 4.0 nm 0 Dmax: 13.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Accessory colonization factor DAMMIN model
Synchrotron SAXS data from solutions of SslE in 20 mM citrate-phosphate buffer, 200 mM NaCl, pH 4.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 10 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403 column at 25°C. 620 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Accessory colonization factor (SslE)
Mol. type   Protein
Organism   Escherichia coli (strain ATCC 9637 / CCM 2024 / DSM 1116 / NCIMB 8666 / NRRL B-766 / W)
Olig. state   Monomer
Mon. MW   160.0 kDa
 
UniProt   E0IW31 (90-1520)
Sequence   FASTA