The C-Terminal Random Coil Region Tunes the Ca2+-Binding Affinity of S100A4 through Conformational Activation

Duelli A, Kiss B, Lundholm I, Bodor A, Petoukhov M, Svergun D, Nyitray L, Katona G, Csernoch L, PLoS ONE 9(5):e97654 (2014) DOI

SASDLW4 – Human Protein S100-A4

Protein S100-A4
MWexperimental 24 kDa
MWexpected 23 kDa
VPorod 48 nm3
log I(s) 2.45×101 2.45×100 2.45×10-1 2.45×10-2
Protein S100-A4 small angle scattering data  s, nm-1
ln I(s)
Protein S100-A4 Guinier plot ln 2.46×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Protein S100-A4 Kratky plot 1.104 0 √3 sRg
p(r)
Protein S100-A4 pair distance distribution function Rg: 2.1 nm 0 Dmax: 7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Protein S100-A4 EOM/RANCH model
Protein S100-A4 EOM/RANCH model
Synchrotron SAXS data from solutions of Human Protein S100-A4 in 20 mM HEPES, 20 mM NaCl, 0.1 mM TCEP, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.0931 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample concentration: UNKNOWN.

Protein S100-A4 (S100A4)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   11.7 kDa
 
UniProt   P26447 (1-101)
Sequence   FASTA