Structural and Functional Characterization of Rv0792c from Mycobacterium tuberculosis: Identifying Small Molecule Inhibitor against HutC Protein.

Chauhan NK, Anand A, Sharma A, Dhiman K, Gosain TP, Singh P, Singh P, Khan E, Chattopadhyay G, Kumar A, Sharma D, Ashish, Sharma TK, Singh R, Microbiol Spectr :e0197322 (2022) Europe PMC

SASDLX8 – SS DNA Aptamer 1 which binds Mtb GntR Homolog [Rv0792c_1]

SSDNA Aptamer from SELEX against Mtb Rv0792c
MWexperimental 14 kDa
MWexpected 13 kDa
log I(s) 7.31×102 7.31×101 7.31×100 7.31×10-1
SSDNA Aptamer from SELEX against Mtb Rv0792c small angle scattering data  s, nm-1
ln I(s)
SSDNA Aptamer from SELEX against Mtb Rv0792c Guinier plot ln 7.31×102 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
SSDNA Aptamer from SELEX against Mtb Rv0792c Kratky plot 1.104 0 √3 sRg
p(r)
SSDNA Aptamer from SELEX against Mtb Rv0792c pair distance distribution function Rg: 2.1 nm 0 Dmax: 8.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
SSDNA Aptamer from SELEX against Mtb Rv0792c CHIMERA model
log I(s)
 s, nm-1
SSDNA Aptamer from SELEX against Mtb Rv0792c DAMMIN model
SSDNA Aptamer from SELEX against Mtb Rv0792c OTHER model
SAXS data from solutions of SS DNA Aptamer 1 which binds Mtb GntR Homolog [Rv0792c_1] in 25 mM HEPES Buffer; 400 mM NaCl, pH 7.2 were collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) using a Mythen 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.15414 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 10°C. Three successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SSDNA Aptamer from SELEX against Mtb Rv0792c (Rv0792c_1)
Mol. type   DNA
Organism   unidentified
Olig. state   Monomer
Mon. MW   13.2 kDa
Sequence   FASTA