Synchrotron SAXS data from solutions of the cytoplasmic domain of diadenylate cyclase from Bacillus subtilis in 30 mM Tris, 150 mM NaCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 0.5 mg/ml was injected at a 0.07 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 15°C. 620 successive 3 second frames were collected through the entire elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the SEC-peak sample scattering frames.
s, nm-1
