| MWI(0) | 165 | kDa |
| MWexpected | 220 | kDa |
| VPorod | 254 | nm3 |
|
log I(s)
3.19×10-2
3.19×10-3
3.19×10-4
3.19×10-5
|
s, nm-1
|
Structural insights into the contactin 1 – neurofascin 155 adhesion complex
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B, Nature Communications 13(1) (2022) DOISASDLY6 – High mannose glycan contactin 1 ectodomain, 2.4 μM
Contactin-1 I433V|
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Fits and models
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Synchrotron SAXS
data from solutions of
High mannose glycan contactin 1 ectodomain, 2.4 μM
in
25 mM HEPES, 150 mM NaCl, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.26 mg/ml was measured.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
High mannose glycan contactin 1 ectodomain 2.4μM. Experimental molecular weight=Sequence Expected MW + MW for 6 N glycosylation sites confirmed crystallographically. X-ray wavelength: UNKNOWN. X-ray exposure time: UNKNOWN. Experimental temperature: UNKNOWN. Dmax severely underestimated. |
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