Coat Protein-Dependent Behavior of Poly(ethylene glycol) Tails in Iron Oxide Core Virus-like Nanoparticles.

Malyutin AG, Cheng H, Sanchez-Felix OR, Carlson K, Stein BD, Konarev PV, Svergun DI, Dragnea B, Bronstein LM, ACS Appl Mater Interfaces 7(22):12089-98 (2015) Europe PMC

SASDM49 – Iron oxide nanoparticles (NP-N3) encapsulated into hepatitis B virus (HBV)

Iron oxide nanoparticles (NP-N3) encapsulated into hepatitis B virus (HBV)

MW^experimental	800	kDa
MW^expected	5000	kDa

log I(s) 5.00×10³ 5.00×10² 5.00×10¹ 5.00×10⁰

Iron oxide nanoparticles (NP-N3) encapsulated into hepatitis B virus (HBV) small angle scattering data

s, nm^-1

Log plot Log-Log plot

(sR_g)²I(s)/I(0)

Iron oxide nanoparticles (NP-N3) encapsulated into hepatitis B virus (HBV) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 9.5 nm 0 D_max: 25.5 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

2.0

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.813
6.151

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Iron oxide nanoparticles (NP-N3) encapsulated into hepatitis B virus (HBV) OTHER [STATIC IMAGE] model

Synchrotron SAXS data from solutions of Iron oxide nanoparticles (NP-N3) encapsulated into hepatitis B virus (HBV) in 0.5 M LiCl, 50 mM HEPES, 2 mM DTT, pH 7.5, pH 7.5 were collected on the EMBL X33 beam line at DORIS III (DESY, Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 10°C. Seven successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The picture shows the transmission electron microscopy image of the nanoparticles. The data have been scaled for display purposes (original data available in the entry archive.)

Tags: X33

Iron oxide nanoparticles (NP-N3) encapsulated into hepatitis B virus (HBV) (NP-N3-HBV)
Mol. type		Other
Olig. state		0
Mon. MW		200 kDa
Chemical formula