Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets.

Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S, mBio :e0063823 (2023) Europe PMC

SASDM65 – Candida albicans Ras-like protein 1

Ras-like protein 1
MWI(0) 22 kDa
MWexpected 33 kDa
VPorod 56 nm3
log I(s) 2.12×102 2.12×101 2.12×100 2.12×10-1
Ras-like protein 1 small angle scattering data  s, nm-1
ln I(s)
Ras-like protein 1 Guinier plot ln 2.13×102 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Ras-like protein 1 Kratky plot 1.104 0 √3 sRg
p(r)
Ras-like protein 1 pair distance distribution function Rg: 4.1 nm 0 Dmax: 15.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Candida albicans Ras-like protein 1 Rg histogram Rg, nm
log I(s)
 s, nm-1
Ras-like protein 1 DAMMIF model
Synchrotron SAXS data from solutions of the Candida albicans Ras-like protein 1(GTP-binding domain and hypervariable region) in 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 5 mM MgCl2, 3 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at Petra-III (DESY, Hamburg, Germany) using a Pilatus 6M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ = 0.124 nm). Different solute concentrations in the range 2.12-16.96 mg/ml were measured using an exposure time of 3 s (recorded as 30 x 0.1 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration and then extrapolated to infinite dilution to simulate a zero-concentration scattering curve. EOM analysis of the flexibility in the hypervariable region. The full EOM results are available in the full entry zip archive.

Storage temperature = UNKNOWN

Ras-like protein 1
Mol. type   Protein
Organism   Candida albicans
Olig. state   Monomer
Mon. MW   32.6 kDa
 
UniProt   P0CY32 (2-290)
Sequence   FASTA