Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets.

Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S, mBio :e0063823 (2023) Europe PMC

SASDM75 – Ras-like protein 1 guanine nucleotide exchange factor region of Candida albicans cell division control protein 25

Cell division control protein 25
MWI(0) 43 kDa
MWexpected 55 kDa
VPorod 81 nm3
log I(s) 3.62×102 3.62×101 3.62×100 3.62×10-1
Cell division control protein 25 small angle scattering data  s, nm-1
ln I(s)
Cell division control protein 25 Guinier plot ln 3.63×102 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Cell division control protein 25 Kratky plot 1.104 0 √3 sRg
p(r)
Cell division control protein 25 pair distance distribution function Rg: 3.3 nm 0 Dmax: 11.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cell division control protein 25 PDB (PROTEIN DATA BANK) model
Cell division control protein 25 DAMMIF model
Synchrotron SAXS data from solutions of the Ras-like protein 1 guanine nucleotide exchange factor region of Candida albicans cell division protein 25 in 20 mM Na-phosphate, 150 mM NaCl, 5% glycerol, 3 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at Petra-III (DESY, Hamburg, Germany) using a Pilatus 6M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ = 0.124 nm). Different solute concentrations in the range 1.39-11.15 mg/ml were measured using an exposure time of 3 s (recorded as 30 x 0.1 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration and then extrapolated to infinite dilution to simulate a zero-concentration scattering curve.

Storage temperature = UNKNOWN

Cell division control protein 25
Mol. type   Protein
Organism   Candida albicans (strain SC5314 / ATCC MYA-2876)
Olig. state   Monomer
Mon. MW   54.9 kDa
 
UniProt   P43069 (860-1333)
Sequence   FASTA
 
PDB ID   7NZZ