Dependence of concentration of precursor clusters formed in lysozyme crystallization solutions on degree of supersaturation and its effect on character of solution transition from liquid to condensed phase

Petr Konarev.

SASDM79 – Lysozyme crystallization solutions with precipitants from crystallization kits CS 1 and CS2 (mixture of monomers, dimers and octamers, with octamer volume fractions from 0 to 0.7%)

Lysozyme C
MWexperimental 22 kDa
MWexpected 14 kDa
log I(s) 2.03×10-2 2.03×10-3 2.03×10-4 2.03×10-5
Lysozyme C small angle scattering data  s, nm-1
ln I(s)
Lysozyme C Guinier plot ln 2.03×10-2 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
Lysozyme C Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Lysozyme C PDB (PROTEIN DATA BANK) model
Lysozyme C PDB (PROTEIN DATA BANK) model
Lysozyme C PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of lysozyme crystallization solutions with precipitants from crystallization kits in 100 mM HEPES pH 7.5, 20 %(v/v) jeffamine M-600, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 20.00 mg/ml was measured at 20°C. 20 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Experimental SAXS data set from lysozyme crystallization solutions with precipitants from crystallization kits CS1 and CS2. The oligomeric composition (volume fractions of monomers, dimers, and octamers) were estimated by OLIGOMER. Volume fractions of octamers are within 0 and 0.7 %. An additional zip file made available in the full entry archive contains the OLIGOMER fit files, volume fractions and a Table key with information about the buffer composition.

Lysozyme C (lysozyme)
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Monomer
Mon. MW   14.3 kDa
 
UniProt   P00698 (19-129)
Sequence   FASTA
 
PDB ID   1LYS