Rigidity, conformation, and solvation of native and oxidized tannin macromolecules in water-ethanol solution.

Zanchi D, Konarev PV, Tribet C, Baron A, Svergun DI, Guyot S, J Chem Phys 130(24):245103 (2009) Europe PMC

SASDM83 – Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%)

Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%)
MWexperimental 2 kDa
MWexpected 2 kDa
log I(s) 9.39×10-3 9.39×10-4 9.39×10-5 9.39×10-6
Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%) small angle scattering data  s, nm-1
ln I(s)
Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%) Guinier plot ln 9.39×10-3 Rg: 1.0 nm 0 (1.0 nm)-2 s2
(sRg)2I(s)/I(0)
Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%) Kratky plot 1.104 0 √3 sRg
p(r)
Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%) pair distance distribution function Rg: 1.0 nm 0 Dmax: 3.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%) DAMMIN model
Synchrotron SAXS data from solutions of Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%), pH 7 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 25°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Native tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 60%) (DP7w60)
Mol. type   Other
Olig. state   Heptamer
Mon. MW   0.3 kDa
Chemical formula