Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets.

Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S, mBio :e0063823 (2023) Europe PMC

SASDM85 – GTP-binding domain of Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25

GTP-binding domain of Ras-like protein 1
Cell division control protein 25
MWI(0) 54 kDa
MWexpected 74 kDa
VPorod 99 nm3
log I(s) 4.66×102 4.66×101 4.66×100 4.66×10-1
GTP-binding domain of Ras-like protein 1 Cell division control protein 25 small angle scattering data  s, nm-1
ln I(s)
GTP-binding domain of Ras-like protein 1 Cell division control protein 25 Guinier plot ln 4.66×102 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
GTP-binding domain of Ras-like protein 1 Cell division control protein 25 Kratky plot 1.104 0 √3 sRg
p(r)
GTP-binding domain of Ras-like protein 1 Cell division control protein 25 pair distance distribution function Rg: 3.3 nm 0 Dmax: 11.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
GTP-binding domain of Ras-like protein 1 Cell division control protein 25 PDB (PROTEIN DATA BANK) model
GTP-binding domain of Ras-like protein 1 Cell division control protein 25 DAMMIF model
Synchrotron SAXS data from solutions of the GTP-binding domain of Candida albicans Ras-like protein 1 in complex with the Ras-like protein 1 guanine nucleotide exchange factor region of cell division control protein 25 in 20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH 7.5 were collected on EMBL P12 beam line at Petra-III (DESY, Hamburg, Germany) using a Pilatus 6M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ = 0.124 nm). Different solute concentrations in the range 1.07-8.53 mg/ml were measured using an exposure time of 3 s (recorded as 30 x 0.1 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration and then extrapolated to infinite dilution to simulate a zero-concentration scattering curve.

Storage temperature = UNKNOWN

GTP-binding domain of Ras-like protein 1
Mol. type   Protein
Olig. state   Monomer
Mon. MW   19.0 kDa
 
UniProt   P0CY32 (2-166)
Sequence   FASTA
 
Cell division control protein 25
Mol. type   Protein
Organism   Candida albicans (strain SC5314 / ATCC MYA-2876)
Olig. state   Monomer
Mon. MW   54.9 kDa
 
UniProt   P43069 (860-1333)
Sequence   FASTA
 
PDB ID   7NZZ