| MWI(0) | 0 | kDa |
| MWexpected | 100 | kDa |
| VPorod | 97 | nm3 |
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log I(s)
9.78×101
9.78×100
9.78×10-1
9.78×10-2
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s, nm-1
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Structural and functional properties of mouse proNGF.
Paoletti F, Konarev PV, Covaceuszach S, Schwarz E, Cattaneo A, Lamba D, Svergun DI, Biochem Soc Trans 34(Pt 4):605-6 (2006) Europe PMCSASDM87 – Mouse ProNGF - pro-form of the NGF (nerve growth factor)
Mouse ProNGF - pro-form of the NGF (nerve growth factor)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Mouse ProNGF - pro-form of the NGF (nerve growth factor)
in
50 mM Na-phosphate, pH 7
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.4 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 6.4 and 12.8 mg/ml were measured
at 25°C.
Two successive
120 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Tags:
X33
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