| MWI(0) | 77 | kDa |
| MWexpected | 87 | kDa |
| VPorod | 149 | nm3 |
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log I(s)
6.95×102
6.95×101
6.95×100
6.95×10-1
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s, nm-1
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Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets.
Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S, mBio :e0063823 (2023) Europe PMCSASDM95 – Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25
Ras-like protein 1Cell division control protein 25
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of the Candida albicans Ras-like protein 1 (GTP-binding domain and hypervariable region) in complex with the guanine nucleotide exchange factor region of cell division control protein 25 in 20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at Petra-III (DESY, Hamburg, Germany) using a Pilatus 6M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ = 0.124 nm). Different solute concentrations in the range 1.41-11.27 mg/ml were measured using an exposure time of 3 s (recorded as 30 x 0.1 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration and then extrapolated to infinite dilution to simulate a zero-concentration scattering curve. EOM analysis of the flexibility in the hypervariable region. The full EOM results are available in the full entry zip archive.
Storage temperature = UNKNOWN |
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