Structural basis for SHOC2 modulation of RAS signalling.

Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J, Nature (2022) Europe PMC

SASDMB5 – Leucine-rich repeat protein SHOC-2

Leucine-rich repeat protein SHOC-2

MW^experimental	58	kDa
MW^expected	65	kDa
V^Porod	85	nm³

log I(s) 1.22×10² 1.22×10¹ 1.22×10⁰ 1.22×10^-1

Leucine-rich repeat protein SHOC-2 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Leucine-rich repeat protein SHOC-2 Guinier plot

ln 1.22×10² R_g: 3.2 nm 0 (3.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

Leucine-rich repeat protein SHOC-2 Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 3.4 nm 0 D_max: 11.2 nm

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Leucine-rich repeat protein SHOC-2 BILBOMD model

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of SHOC-2 in 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 28 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex LW-803 column at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Leucine-rich repeat protein SHOC-2
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Monomer
Mon. MW		65.1 kDa

UniProt		Q9UQ13 (2-582)
Sequence		FASTA