| MWexperimental | 105 | kDa |
| MWexpected | 121 | kDa |
| VPorod | 156 | nm3 |
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log I(s)
5.52×101
5.52×100
5.52×10-1
5.52×10-2
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s, nm-1
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Structural basis for SHOC2 modulation of RAS signalling.
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J, Nature (2022) Europe PMCSASDMD5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) and GTPase KRas
Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
GTPase KRas
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Fits and models
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Synchrotron SAXS data from solutions of SHOC-2-PP1C-KRas in 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 55 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex LW-803 column at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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