A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA.

Pham VV, Gao M, Meagher JL, Smith JL, D'Souza VM, Commun Biol 5(1):819 (2022) Europe PMC

SASDMD9 – 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat Group M, Subtype G

Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA
Protein Tat
MWexperimental 20 kDa
MWexpected 20 kDa
VPorod 28 nm3
log I(s) 3.87×101 3.87×100 3.87×10-1 3.87×10-2
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein Tat small angle scattering data  s, nm-1
ln I(s)
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein Tat Guinier plot ln 3.87×101 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein Tat Kratky plot 1.104 0 3 sRg
p(r)
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein Tat pair distance distribution function Rg: 2.6 nm 0 Dmax: 9.9 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat in 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH 5.6 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 33 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Concentration: UNKNOWN.

Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA (7SK RNA stem loop-1)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   18.1 kDa
Sequence   FASTA
 
Protein Tat (HIV-1 Tat)
Mol. type   Protein
Organism   Human immunodeficiency virus type 1 group M subtype G (isolate SE6165)
Olig. state   Monomer
Mon. MW   2.2 kDa
 
UniProt   O89943 (44-60)
Sequence   FASTA