A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA.

Pham VV, Gao M, Meagher JL, Smith JL, D'Souza VM, Commun Biol 5(1):819 (2022) Europe PMC

SASDME9 – 7SK stem-loop 1 with HEXIM Arginine Rich Motif

Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA
Protein HEXIM1
MWexperimental 17 kDa
MWexpected 20 kDa
VPorod 26 nm3
log I(s) 1.33×101 1.33×100 1.33×10-1 1.33×10-2
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein HEXIM1 small angle scattering data  s, nm-1
ln I(s)
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein HEXIM1 Guinier plot ln 1.33×101 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein HEXIM1 Kratky plot 1.104 0 √3 sRg
p(r)
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein HEXIM1 pair distance distribution function Rg: 2.4 nm 0 Dmax: 8.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of 7SK stem-loop 1 with HEXIM Arginine Rich Motif in 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH 5.6 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 33 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The HEXIM1 peptide has adapter on either side of the HEXIM arginine rich motif as matched to the HIV-1 Tat Subtype B from the following study: Pham, V.V., Salguero, C., Khan, S.N. et al. HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry. Nat Commun 9, 4266 (2018). https://doi.org/10.1038/s41467-018-06591-6. Concentration: UNKNOWN.

Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA (7SK RNA stem loop-1)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   18.1 kDa
Sequence   FASTA
 
Protein HEXIM1 (HEXIM1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   2.3 kDa
 
UniProt   O94992 (146-156)
Sequence   FASTA