Rigidity, conformation, and solvation of native and oxidized tannin macromolecules in water-ethanol solution.

Zanchi D, Konarev PV, Tribet C, Baron A, Svergun DI, Guyot S, J Chem Phys 130(24):245103 (2009) Europe PMC

SASDMF3 – Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%)

Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%)

MW^experimental	2	kDa
MW^expected	2	kDa

log I(s) 1.95×10¹ 1.95×10⁰ 1.95×10^-1 1.95×10^-2

$Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%) small angle scattering data$ s, nm^-1

Log plot Log-Log plot

ln I(s)

$Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%) Guinier plot$ ln 1.96×10¹ R_g: 1.2 nm 0 (1.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

$Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%) Kratky plot$ 1.104 0 √3 sR_g

p(r)	$Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%) pair distance distribution function$ R_g: 1.2 nm 0 D_max: 3.6 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.7

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.001
4.060

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

$Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%) DAMMIN model$

Synchrotron SAXS data from solutions of Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%), pH 7 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 25°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33

Oxidized tannin macromolecules (DP7, average polymerization 6.3) in water-ethanol solution (water fraction 80%) (DP7oxw80)
Mol. type		Other
Olig. state		Heptamer
Mon. MW		0.3 kDa
Chemical formula