A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA.

Pham VV, Gao M, Meagher JL, Smith JL, D'Souza VM, Commun Biol 5(1):819 (2022) Europe PMC

SASDMF9 – 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat Group M, Subtype B (Isolate A18188)

Protein Tat
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA
MWexperimental 19 kDa
MWexpected 20 kDa
VPorod 28 nm3
log I(s) 7.73×100 7.73×10-1 7.73×10-2 7.73×10-3
Protein Tat Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA small angle scattering data  s, nm-1
ln I(s)
Protein Tat Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Guinier plot ln 7.74×100 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Protein Tat Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Kratky plot 1.104 0 √3 sRg
p(r)
Protein Tat Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA pair distance distribution function Rg: 2.6 nm 0 Dmax: 9.7 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat in 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH 5.6 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 33 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

This protein is an exact match to Genbank ID: UEP57589.1. It is a Tat protein derived from HIV-1 Group M, Subtype B (isolate A18188). Refer to https://www.ncbi.nlm.nih.gov/protein/UEP57589.1. Concentration: UNKNOWN.

Protein Tat (HIV-1 Tat)
Mol. type   Protein
Organism   Human immunodeficiency virus type 1, Group M, Subtype B (isolate A18188)
Olig. state   Monomer
Mon. MW   2.2 kDa
Sequence   FASTA
 
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA (7SK RNA stem loop-1)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   18.1 kDa
Sequence   FASTA