Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori.

Tamrakar A, Singh R, Kumar A, Makde RD, Ashish, Kodgire P, Sci Rep 11(1):24471 (2021) Europe PMC

SASDMK7 – HomA outer membrane protein

HomA outer membrane protein
MWexperimental 170 kDa
MWexpected 146 kDa
VPorod 426 nm3
log I(s) 1.94×10-1 1.94×10-2 1.94×10-3 1.94×10-4
HomA outer membrane protein small angle scattering data  s, nm-1
ln I(s)
HomA outer membrane protein Guinier plot ln 1.94×10-1 Rg: 8.3 nm 0 (8.3 nm)-2 s2
(sRg)2I(s)/I(0)
HomA outer membrane protein Kratky plot 1.104 0 √3 sRg
p(r)
HomA outer membrane protein pair distance distribution function Rg: 8.3 nm 0 Dmax: 28.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
HomA outer membrane protein DAMMIN model
Synchrotron SAXS data from solutions of HomA protein in 20 mM Tris-HCl, 200 mM NaCl 5mM BME, pH 8 were collected on the BL-18 beam line at INDUS-2 (Indore, India) using a MAR 345 Image Plate detector at a sample-detector distance of 3.2 m and at a wavelength of λ = 0.07749 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.5 mg/ml was measured at 37°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

CAUTION! Sample is likely aggregated. CAUTION! Guinier region ill-defined. CAUTION! Scattering intensity errors are over-estimated. CAUTION! Model likely does not represent a single particle.

HomA outer membrane protein (HomA)
Mol. type   Protein
Organism   Helicobacter pylori
Olig. state   Dimer
Mon. MW   72.9 kDa
Sequence   FASTA