Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase.

Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME, Biophys J (2022) Europe PMC

SASDMK8 – Sulfite reductase flavoprotein octamer

Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)

MW^experimental	578	kDa
MW^expected	565	kDa
V^Porod	830	nm³

log I(s) 1.21×10⁰ 1.21×10^-1 1.21×10^-2 1.21×10^-3

Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Guinier plot

ln 1.22×10⁰ R_g: 8.6 nm 0 (8.6 nm)^-2 s²

(sR_g)²I(s)/I(0)

Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 8.1 nm 0 D_max: 26 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

1.0

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.287
0.787

Worse

Better

There are no models related to this curve.

SANS data from solutions of sulfite reductase flavoprotein octamer in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). This sample is an octamer of His-tagged sulfite reductase flavoprotein subunits. Three instrument configurations were used: 9 m sample-to-detector distance with 1.5 nm wavelength band, 4 m sample-to-detector distance with 0.6 nm wavelength band, and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 3 mg/mL. Neutron exposure time: 3 hours / instrument configuration. Sample temperature: 8°C.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) (His-tagged SiRFP)
Mol. type		Protein
Organism		Escherichia coli (strain K12)
Olig. state		Octamer
Mon. MW		70.7 kDa

UniProt		P38038 (1-590)
Sequence		FASTA