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Synchrotron SAXS
data from solutions of
LAF + MNP (r = 5.6 nm) nanocomposite
in
0.2 M glycine-HCl, 80 mM NaCl, pH 2.2
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 4.2 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
at 20°C.
20 successive
0.100 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Magnetite nanoparticles were fabricated by the coprecipitation of Fe2+ and Fe3+ by NH4OH at 60 °C. Afterwards, an magnetic fluid (MF) was obtained by dispersion of the freshly prepared magnetite nanoparticles in water and electrostatically stabilized by the addition of HClO4. The final concentration of MNPs in the MF was 28 mg/mL. The hydrodynamic diameter of the MNPs, determined by dynamic light scattering (DLS) (Zetasizer), has a nominal size of 26 nm alongside polydispersity of 0.26, and the radius of MNPs estimated from AFM was 5.6 nm.
Theoretical SAXS profile computed by DAMMIX analysis of a concentration series from LAF and MNP (r = 5.6 nm) nanoparticles (in air SAXS). SVD analysis showed the presence of a third component in the mixture, which was identified as this nanocomposite.
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s, nm-1
