| MWexperimental | 15 | kDa |
| MWexpected | 29 | kDa |
| VPorod | 18 | nm3 |
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log I(s)
1.57×101
1.57×100
1.57×10-1
1.57×10-2
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s, nm-1
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A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence.
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR, Nat Commun 13(1):4105 (2022) Europe PMCSASDMM3 – Mycobacterium phage TipsytheTRex immunity repressor mixed with 13mer DNA
Immunity repressor13mer dsDNA
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Fits and models
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Synchrotron SAXS data from solutions of Mycobacterium phage TipsytheTRex immunity repressor mixed with 13mer DNA in 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The SAXS data is best described as a mixture of 64% free DNA and 36% free protein (i.e., the protein and DNA do not form a complex). |
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