Structural Insights into the Regulatory Mechanism of the Response Regulator RocR from Pseudomonas aeruginosa in Cyclic Di-GMP Signaling

Chen M, Kotaka M, Vonrhein C, Bricogne G, Rao F, Chuah M, Svergun D, Schneider G, Liang Z, Lescar J, Journal of Bacteriology 194(18):4837-4846 (2012) DOI

SASDMM9 – Response Regulator RocR from Pseudomonas aeruginosa

RocR
MWexperimental 170 kDa
MWexpected 171 kDa
log I(s) 1.06×102 1.06×101 1.06×100 1.06×10-1
RocR small angle scattering data  s, nm-1
ln I(s)
RocR Guinier plot ln 1.07×102 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
RocR Kratky plot 1.104 0 √3 sRg
p(r)
RocR pair distance distribution function Rg: 3.6 nm 0 Dmax: 11 nm

Data validation

Fits and models

log I(s)
 s, nm-1
RocR OTHER model
log I(s)
 s, nm-1
RocR DAMMIF model
Synchrotron SAXS data from solutions of Response Regulator RocR from Pseudomonas aeruginosa in 50 mM Tris–HCl, 250 mM NaCl, 10 mM imidazole, 5% glycerol, 0.5 mM DTT, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.50 mg/ml was measured. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

Tags: X33
RocR
Mol. type   Protein
Organism   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Olig. state   Tetramer
Mon. MW   42.8 kDa
 
UniProt   Q9HX69
Sequence   FASTA