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Synchrotron SAXS data from solutions of LRSAM1 in 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM TCEP, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 240.00 μl sample at 4.8 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 200 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The models displayed show both the average-weighted bead-occupancy and volume-corrected representation of the protein calculated from the spatial alignment of a cohort of individual models (damfilt; top) and an individual model reconstruction (bottom) with the associated fit to the data.
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s, nm-1
