Leucine Rich Repeat And Sterile Alpha Motif Containing 1 (LRSAM1)

Si Hoon Park.

SASDMP3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (1-632): C-terminal PTAP/PSAP motif and RING domain deletion mutant, ΔPTAP/PSAP-RING

E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING
MWexperimental 208 kDa
MWexpected 74 kDa
VPorod 322 nm3
log I(s) 1.16×101 1.16×100 1.16×10-1 1.16×10-2
E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING small angle scattering data  s, nm-1
ln I(s)
E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING Guinier plot ln 1.16×101 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING Kratky plot 1.104 0 √3 sRg
p(r)
E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING pair distance distribution function Rg: 5.6 nm 0 Dmax: 18 nm

Data validation

Fits and models

log I(s)
 s, nm-1
E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING DAMFILT model
E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING DAMMIN model
Synchrotron SAXS data from solutions of LRSAM1 (amino acids 1-632) in 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 1 mM TCEP, 5% w/v glycerol, pH 8 were collected on the 4C beam line at the Pohang Accelerator Laboratory (Pohang, South Korea) using a Rayonix SX165 detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.0734 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.20 mg/ml was measured at 20°C. Six successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The models displayed show both the average-weighted bead-occupancy and volume-corrected representation of the protein calculated from the spatial alignment of a cohort of individual models (damfilt; top) and an individual model reconstruction (bottom) with the associated fit to the data.

E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING (LRSAM1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   73.6 kDa
 
UniProt   Q6UWE0 (1-632)
Sequence   FASTA