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Synchrotron SAXS data from solutions of 5-10-5 gapmer phosphorothioate antisense oligonucleotide in 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample at 7.1 mg/ml was injected at a 0.50 ml/min flow rate onto a Wyatt WTC-030S5 5μm 300+, 7.8x300 mm column at 4°C. 900 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The ab initio bead models presented in this entry include: 1) The spatially aligned and volume/bead occupancy-corrected averaged representation of the 5-10-5 gapmer phosphorothioate antisense oligonucleotide calculated from a individual model cohort (top) and; 2) An example of an individual model reconstruction (bottom) and the corresponding fit to the data.
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s, nm-1
