Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase.

Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME, Biophys J (2022) Europe PMC

SASDMR8 – Reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 100% D2O

Sulfite reductase [NADPH] hemoprotein beta-component
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
MWexperimental 839 kDa
MWexpected 821 kDa
log I(s) 9.02×10-1 9.02×10-2 9.02×10-3 9.02×10-4
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Guinier plot ln 9.03×10-1 Rg: 8.5 nm 0 (8.5 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Kratky plot 1.104 0 √3 sRg
p(r)
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) pair distance distribution function Rg: 8.3 nm 0 Dmax: 25.1 nm

Data validation

There are no models related to this curve.

SANS data from solutions of reconstituted sulfite reductase dodecamer with partially deuterated hemoprotein in 100% D2O, 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). Three instrument configurations used were: 9 m sample-to-detector distance with 1.5 nm wavelength band, 4 m sample-to-detector distance with 0.6 nm wavelength band, and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 4.2 mg/mL. Neutron exposure time: 3 hours / instrument configuration. Sample temperature: 8°C.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Sulfite reductase [NADPH] hemoprotein beta-component (SiRHP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Tetramer
Mon. MW   64.0 kDa
 
UniProt   P17846 (1-570)
Sequence   FASTA
 
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) (His-tagged SiRFP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   70.7 kDa
 
UniProt   P38038 (1-590)
Sequence   FASTA