X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB

Niemann H, Petoukhov M, Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D, Journal of Molecular Biology 377(2):489-500 (2008) DOI

SASDMS4 – Listeria monocytogenes invasion protein internalin B

Internalin B
MWexperimental 33 kDa
MWexpected 36 kDa
VPorod 54 nm3
log I(s) 2.36×102 2.36×101 2.36×100 2.36×10-1
Internalin B small angle scattering data  s, nm-1
ln I(s)
Internalin B Guinier plot ln 2.37×102 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Internalin B Kratky plot 1.104 0 √3 sRg
p(r)
Internalin B pair distance distribution function Rg: 2.9 nm 0 Dmax: 9.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Internalin B PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Listeria monocytogenes invasion protein internalin B in 25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a 1D Gas detector detector at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 32.00 mg/ml was measured. 15 successive 60 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
Internalin B (InlB)
Mol. type   Protein
Organism   Listeria monocytogenes serotype 1/2a (strain EGD / Mackaness)
Olig. state   Monomer
Mon. MW   35.8 kDa
 
UniProt   P0DQD3 (1-320)
Sequence   FASTA
 
PDB ID   2UZX