A MademoiseLLE domain binding platform links the key RNA transporter to endosomes

Devan S, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A, PLOS Genetics 18(6):e1010269 (2022) DOI

SASDMS5 – Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion

RNA recognition motif (RRM)-containing protein 4

MW^I(0)	104	kDa
MW^expected	111	kDa
V^Porod	587	nm³

log I(s) 1.04×10² 1.04×10¹ 1.04×10⁰ 1.04×10^-1

RNA recognition motif (RRM)-containing protein 4 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

RNA recognition motif (RRM)-containing protein 4 Guinier plot

ln 1.04×10² R_g: 8.8 nm 0 (8.8 nm)^-2 s²

(sR_g)²I(s)/I(0)

RNA recognition motif (RRM)-containing protein 4 Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 9.0 nm 0 D_max: 30.7 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.5

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.097
1.016

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion Rg histogram

R_g, nm

RNA recognition motif (RRM)-containing protein 4 EOM/RANCH model

Synchrotron SAXS data from solutions of GST-Rrm4 in 20 mM Hepes, 200 mM NaCl, 1 mM βME, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

RNA recognition motif (RRM)-containing protein 4 (GST_Rrm4)
Mol. type		Protein
Organism		Ustilago maydis
Olig. state		Monomer
Mon. MW		111.0 kDa

UniProt		A0A0D1DWZ5 (4-792)
Sequence		FASTA