| MWexperimental | 62 | kDa |
| MWexpected | 60 | kDa |
| VPorod | 96 | nm3 |
|
log I(s)
2.78×10-2
2.78×10-3
2.78×10-4
2.78×10-5
|
s, nm-1
|
Structural basis of dimerization and nucleic acid binding of human DBHS proteins NONO and PSPC1.
Knott GJ, Chong YS, Passon DM, Liang XH, Deplazes E, Conte MR, Marshall AC, Lee M, Fox AH, Bond CS, Nucleic Acids Res (2021) Europe PMCSASDMS6 – Non-POU domain-containing octamer-binding protein (NONO homodimer)
Non-POU domain-containing octamer-binding protein|
|
|
Fits and models
|
|
|
Synchrotron SAXS data from solutions of the NONO homodimer in 20 mM Tris-Cl (pH 7.5), 250 mM KCl, 50 mM L-proline, 0.5 mM EDTA were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample at 7 mg/ml was injected at a 0.50 ml/min flow rate onto a Wyatt WTC-030S5 5μm 300+, 7.8x300 mm column at 4°C. 700 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Homodimer of human nuclear paraspeckle protein NONO also known as p54nrb. Same construct as PDB entry 5IFM. |
|
|||||||||||||||||||||||||||||||||