| MWexperimental | 670 | kDa |
| MWexpected | 29520 | kDa |
|
log I(s)
1.62×102
1.62×101
1.62×100
1.62×10-1
|
s, nm-1
|
Macromolecular HPMA-based nanoparticles with cholesterol for solid-tumor targeting: detailed study of the inner structure of a highly efficient drug delivery system.
Filippov SK, Chytil P, Konarev PV, Dyakonova M, Papadakis C, Zhigunov A, Plestil J, Stepanek P, Etrych T, Ulbrich K, Svergun DI, Biomacromolecules 13(8):2594-604 (2012) Europe PMCSASDMT2 – HPMA-Based Nanoparticles with Cholesterol (3.0%)
N-(2-hydroxypropyl)- 31 methacrylamide (HPMA) copolymers with Cholesterol (3.0%)|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
HPMA-Based Nanoparticles with Cholesterol (3.0%)
in
phosphate buffer saline (PBS) (pH 5.0), pH 5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.00 mg/ml was measured
at 20°C.
Eight successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Tags:
X33
|
|
||||||||||||||||||