Protein quaternary structures in solution are a mixture of multiple forms

Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G, Chemical Science 13(39):11680-11695 (2022) DOI

SASDMT3 – NAD Kinase

NAD kinase
MWI(0) 88 kDa
MWexpected 33 kDa
VPorod 260 nm3
log I(s) 6.34×10-2 6.34×10-3 6.34×10-4 6.34×10-5
NAD kinase small angle scattering data  s, nm-1
ln I(s)
NAD kinase Guinier plot ln 6.35×10-2 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
NAD kinase Kratky plot 1.104 0 √3 sRg
p(r)
NAD kinase pair distance distribution function Rg: 4.0 nm 0 Dmax: 12.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
NAD kinase PDB (PROTEIN DATA BANK) model
NAD kinase PISA model
NAD kinase PISA model
NAD kinase PISA model
Synchrotron SAXS data from solutions of NAD Kinase in 50 mM HEPES, pH 7.2 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. 40 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

NAD kinase (NadK)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Unknown
Mon. MW   32.6 kDa
 
UniProt   P0A7B3 (1-292)
Sequence   FASTA
 
PDB ID   4HAO
 
PDB ID   4HAO
 
PDB ID   4HAO
 
PDB ID   4HAO