A MademoiseLLE domain binding platform links the key RNA transporter to endosomes

Devan S, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A, PLOS Genetics 18(6):e1010269 (2022) DOI

SASDMT5 – N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4

RNA recognition motif (RRM)-containing protein 4 NT4
MWI(0) 43 kDa
MWexpected 40 kDa
VPorod 123 nm3
log I(s) 4.33×101 4.33×100 4.33×10-1 4.33×10-2
RNA recognition motif (RRM)-containing protein 4 NT4 small angle scattering data  s, nm-1
ln I(s)
RNA recognition motif (RRM)-containing protein 4 NT4 Guinier plot ln 4.33×101 Rg: 5.6 nm 0 (5.6 nm)-2 s2
(sRg)2I(s)/I(0)
RNA recognition motif (RRM)-containing protein 4 NT4 Kratky plot 1.104 0 √3 sRg
p(r)
RNA recognition motif (RRM)-containing protein 4 NT4 pair distance distribution function Rg: 5.5 nm 0 Dmax: 18.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4 Rg histogram Rg, nm
RNA recognition motif (RRM)-containing protein 4 NT4 EOM/RANCH model
RNA recognition motif (RRM)-containing protein 4 NT4 EOM/RANCH model
RNA recognition motif (RRM)-containing protein 4 NT4 EOM/RANCH model
Synchrotron SAXS data from solutions of His-Rrm4NT4 in 20 mM Hepes, 200 mM NaCl, 1 mM βME, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 10°C. Eight successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

RNA recognition motif (RRM)-containing protein 4 NT4 (His-Rrm4NT4)
Mol. type   Protein
Organism   Ustilago maydis
Olig. state   Monomer
Mon. MW   40.3 kDa
 
UniProt   A0A0D1DWZ5 (421-792)
Sequence   FASTA